高源;申辛欣;冯志山;马学军;

• 1:河北医科大学研究生学院
• 2:中国疾病预防控制中心病毒病预防控制所卫健委医学病毒与病毒病重点实验室
• 3:河北省人民医院

摘要(Abstract)：

简化核酸提取过程相较于经典的试剂盒法和全自动核酸提取技术,凭借其简便的操作步骤、便携易用的仪器设备和简单的人员培训等优势在病原体核酸检测中得到广泛应用,为实现病原体核酸的快速检测和现场检测(Point-of-care testing,POCT)奠定了良好的基础。本文比较了不同的核酸提取方法,对简化核酸提取过程的方法学进行综述,讨论其在病原体核酸检测领域中的应用现状,并展望其发展趋势。

关键词(KeyWords)： 简化核酸提取过程;核酸检测;现场检测

Abstract:

Keywords:

基金项目(Foundation): 国家传染病重大专项（项目号：2018ZX10711-001、2017ZX10104001、2017ZX10302301-004-002），题目：病毒感染高通量快速检测与应急筛检技术研究，基于全基因组学的病毒网络化监测和朔源技术体系研究，基因重组酶介导的等温扩增（RAA）技术耐药SNP检测方法建立及应用;; 侯云德院士基金项目（2019HYDQN-JJ03），题目：RT-RAA等温检测呼吸道合胞病毒（RSV）核酸试剂盒临床评估研究~~

作者(Author): 高源;申辛欣;冯志山;马学军;

Email:

DOI: 10.13242/j.cnki.bingduxuebao.003745

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