张雪,王传红,钱嘉莉,徐红,宋旭,宝梅英,刘诗雨,胡朝阳,张雪寒,郭容利,贾斌,范宝超,李彬

• 1:石河子大学动物科技学院
• 2:江苏省农业科学院兽医研究所
• 3:江苏大学
• 4:南京农业大学

摘要(Abstract)：

肠黏膜屏障的完整性与猪流行性腹泻病毒（Porcine epidemic diarrhea virus,PEDV）的致病性密切相关。黏蛋白2(Mucin 2,Muc2)作为肠黏膜屏障的重要组成蛋白，在阻止病原体、毒素和异物入侵等方面发挥重要作用。本文旨在探究黏蛋白Muc2对PEDV复制的拮抗作用。通过观察PEDV感染仔猪空肠黏膜屏障情况，利用RNA干扰策略和分子病毒学检测方法在细胞水平上研究分析Muc2表达水平与PEDV复制的关系，并进一步探讨猪源Muc2天然蛋白发挥抗PEDV作用的具体阶段。结果显示：PEDV感染仔猪空肠段进行PAS染色发现，病毒感染后肠绒毛表面黏液层变薄，结构被破坏，杯状细胞明显减少；与对照组相比，干扰Muc2基因能够上调PEDV-N的mRNA及蛋白水平；Muc2蛋白添加后显著抑制PEDV-N蛋白的表达，且病毒mRNA水平显著下降（P<0.05）；在病毒感染前使用50μg/mL Muc2蛋白预处理1 h后，PEDV-N mRNA相对表达量极显著下降（P<0.001），而在病毒吸附、入侵和复制阶段无显著差异（P>0.05），表明Muc2具有降低PEDV侵染宿主细胞的作用。综上所述，本研究验证了Muc2在PEDV感染中的拮抗作用，发现了Muc2主要作用于病毒侵染宿主细胞阶段，为深入探讨Muc2与PEDV间的作用机制奠定了基础，也为制定PEDV新防控策略提供了思路。

关键词(KeyWords)： 猪流行性腹泻病毒;Muc2;肠道屏障

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（项目号：32272996）,题目：PEDV调控基质金属酶13促进病毒吸附的分子机制研究;; 江苏省种业振兴揭榜挂帅项目（项目号：JBGS[2021]024）,题目：猪特色种质资源关键基因挖掘与育种新材料创制~~

作者(Author): 张雪,王传红,钱嘉莉,徐红,宋旭,宝梅英,刘诗雨,胡朝阳,张雪寒,郭容利,贾斌,范宝超,李彬

DOI: 10.13242/j.cnki.bingduxuebao.004358

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