吴铮;苏韫曦;丛鑫;王金冬;朱曦;靳淼;孙晓曼;段招军;

• 1:甘肃中医药大学公共卫生学院
• 2:中国疾病预防控制中心病毒病预防控制所

摘要(Abstract)：

分析GⅡ.4基因型中国香港株（GⅡ.4 HongKong,GⅡ.4 HK）P颗粒蛋白与组织血型抗原的相互作用方式以探索GⅡ.4 HK的受体结合特征。利用大肠杆菌蛋白表达系统得到GⅡ.4 HK及GⅡ.4 Sydney的P颗粒蛋白，通过寡糖和唾液结合实验研究其与不同组织血型抗原的结合特异性；对序列和结构进行分析，比较P蛋白的结构和功能特征。成功制备到GⅡ.4 HK与GⅡ.4 Sydney的P颗粒蛋白，GⅡ.4 HK和GⅡ.4 Sydney P颗粒与人A、B、AB、O型唾液均有结合，也与含fucose的H双糖寡糖有很好的结合。GⅡ.4 HK与GⅡ.4 Sydney流行株具有相似的组织血型抗原结合特征，但结合能力弱于GⅡ.4 Sydney流行株，GII.4 HK在多个抗原位点有氨基酸改变，可能引起抗原改变，有必要对GⅡ.4 HK流行情况的持续监测。

关键词(KeyWords)： 诺如病毒;P颗粒;组织血型抗原;唾液结合;寡糖结合

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划（项目号：2021YFC2301005）,题目：非传统细胞系靶向培养技术集成与应用评价;; 国家自然科学基金（项目号：21934005）,题目：中国母乳糖组高效分离分析及与轮状病毒蛋白相互作用研究~~

作者(Authors): 吴铮;苏韫曦;丛鑫;王金冬;朱曦;靳淼;孙晓曼;段招军;

DOI: 10.13242/j.cnki.bingduxuebao.004224

参考文献(References)：

• [1] Marks PJ, Vipond IB, Carlisle D, Deakin D, Fey RE,Caul EO. Evidence for airborne transmission of Norwalklike virus(NLV)in a hotel restaurant.[J]. Epidemiol and Infect, 2000, 124(3):481-487.
• [2] Verhoef L, Hewitt J, Barclay L, Ahmed SM, Lake R,Hall AJ, Lopman B, Kroneman A, Vennema H, VinjéJ, Koopmans M. Norovirus genotype profiles associated with foodborne transmission, 1999-2012.[J]. Emerg Infect Dis, 2015, 21(4):592-599.
• [3] Wikswo ME, Hall AJ.Outbreaks of acute gastroenteritis transmitted by person-to-person Contact-United States, 2009-2010.[J]. Mmwr Surveillance Summaries,2012.
• [4] Lysén M, Thorhagen M, Brytting M, Hjertqvist M,Andersson Y, Hedlund KO. Genetic diversity among food-borne and waterborne norovirus strains causing outbreaks in Sweden[J]. J Clin Microbiol, 2009, 47(8):2411-2418.
• [5] Chhabra P, de Graaf M, Parra GI, Chan MC, Green K, Martella V, Wang Q, White PA, Katayama K,Vennema H, Koopmans MPG, VinjéJ. Updated classification of norovirus genogroups and genotypes[J].J General Virol, 2019, 100(10).
• [6] Pietsch C, Ennuschat N, H?rtel S, Liebert UG. Withinhost evolution of virus variants during chronic infection with novel GII.P26-GII.26 norovirus[J]. J Clin Virol,2018, 108:96-102.
• [7] Joukje S J,Harry V, Zheng D P,VinjéJ, Lee BE,Pang XL, Ho EC, Lim W, Choudekar A, Broor S,Halperin T, Rasool NB, Hewitt J, Greening GE, Jin M, Duan ZJ, Lucero Y, O'Ryan M, Hoehne M,Schreier E, Ratcliff RM, White PA, Iritani N, Reuter G, Koopmans M. Norovirus illness is a global problem:emergence and spread of norovirus GII.4 variants, 2001-2007[J]. J Infect Dis, 2009, 200(5):802-812.
• [8] Beek J V, Graaf M D, Al-Hello H, Allen DJ, AmbertBalay K, Botteldoorn N, Brytting M, Buesa J,Cabrerizo M, Chan M, Cloak F, Di Bartolo I, Guix S,Hewitt J, Iritani N, Jin M, Johne R, Lederer I, Mans J, Martella V, Maunula L, McAllister G, Niendorf S,Niesters HG, Podkolzin AT, Poljsak-Prijatelj M,Rasmussen LD, Reuter G, Tuite G, Kroneman A,Vennema H, Koopmans MPG. Molecular surveillance of norovirus, 2005–16:an epidemiological analysis of data collected from the NoroNet network[J]. Lancet Infect Dis, 2018:545.
• [9] Chan MC, Roy S, Bonifacio J, Zhang LY, Chhabra P,Chan JCM, Celma C, Igoy MA, Lau SL, Mohammad KN, VinjéJ, Vennema H, Breuer J, Koopmans M, de Graaf M; for NOROPATROL2. Detection of norovirus variant GII.4 Hong Kong in Asia and Europe, 20172019[J]. Emerg Infect Dis, 2021, 27(1):289-293.
• [10]Tse E, Zhang L Y,Lau S L,Chan, C W. Genome sequence of a human norovirus GII.4 Hong Kong[P31]variant in Hong Kong, China[J]. Microbiol Res Announcements, 2020, 9(3).
• [11]Chuchaona W, Chansaenroj J, Puenpa J, Khongwichit S, Korkong S, Vongpunsawad S, Poovorawan Y.Human norovirus GII. 4 Hong Kong variant shares common ancestry with GII. 4 Osaka and emerged in Thailand in 2016[J/OL]. PLoS One. 2021,16(8)
• [12]梁耀民，陆胤波，曹颖雯，罗奕熔，蒋月婷，寇晓霞.诺如病毒和肠道菌群的互作关系及机制研究进展[J/OL].病毒学报，2021, 37(5):6.DOI:10.13242/j.cnki.bingduxuebao.003962.
• [13]Prasad, Vv B. X-ray crystallographic structure of the Norwalk virus capsid[J]. Science, 1999, 286(5438):287-290.
• [14]Jung J,Grant T,Thomas D R, Diehnelt C W,Grigorieff N,Joshua-Tor L. High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations[J]. Proc Natl Acad Sci U S A, 2019,116(26).
• [15]Cong X, Sun X M, Qi J X, Li H B, Chai W G, Zhang Q, Wang H, Kong X Y, Song J, Pang L L, Jin M, Li D D, Tan M, Duan Z J. GII. 13/21 noroviruses recognize glycans with a terminal β-Galactose via an unconventional glycan binding site[J]. J Virol, 2019,93(15).
• [16]丛鑫，李涵博，段招军. GⅡ.23基因型诺如病毒P蛋白的寡糖结合特征[J/OL].病毒学报，2019, 35(1):6.DOI:10.13242/j.cnki.bingduxuebao.003491.
• [17]Singh B K, Leuthold M M, Hansman G S, SandriGoldin R M. Human noroviruses'fondness for histoblood group antigens.[J]. J Virol, 2015, 89(4):2024-40.
• [18]Cannon J L, Barclay L, Collins N R, Wikswo M E,Castro C J, Maga?a L C, Gregoricus N, Marine R L,Chhabra P, Vinjéb J. Genetic and epidemiologic trends of norovirus outbreaks in the united states from 2013 to2016 demonstrated emergence of novel gii.4 recombinant viruses[J]. J Clin Microbiol, 2017, 55(7):JCM.00455-17.