王金昌;类承凤;陈俊晖;占智高;况文东;关丽梅;杨健;李江怀;周溪;靳亮;王君;

• 1:江西省科学院微生物研究所
• 2:中国科学院武汉病毒研究所

摘要(Abstract)：

从自然死亡的扁刺蛾幼虫分离到一株核型多角体病毒。扁刺蛾核型多角体病毒新分离株（Oxyplax ochracea nucleopolyhedrovirus-Thosea sinensis,OxocNPV-Ts）具有典型的杆状病毒特征，呈单粒包埋型病毒粒子，病毒颗粒（Occlusion-derived virus,ODV）呈不规则的多边形，直径为0.63μm～1.49μm。OxocNPV-Ts能有效杀灭扁刺蛾幼虫，它对扁刺蛾幼虫的半致死浓度LC_(50)为4×10⁶ PIB/mL，是一种理想的生物防治剂。本实验对OxocNPV-Ts基因组进行了测序和鉴定。OxocNPV-Ts基因组全长114781 bp，包含124个开放阅读框。它与斜纹刺蛾核型多角体病毒（OxocNPV）关系最为密切，序列相似性高达99%。系统进化分析表明，OxocNPV-Ts属于杆状病毒科alpha杆状病毒属（Alphabaculovirus），根据国际病毒分类委员会（International Committee on Taxonomy of Viruses,ICTV）的种属划分标准，OxocNPV-Ts是OxocNPV的一个株系。新报道的基因组为研究该病毒毒力的分子机制提供了理论基础。

关键词(KeyWords)： 扁刺蛾核型多角体病毒;基因组;测序分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（项目号：31900154）,题目：深度挖掘杆状病毒蛋白质网络;; 江西省重点研发项目（项目号：20192BBF60056）,题目：草原毛虫多角体病毒复配增效杀虫剂关键技术及应用研究;江西省重点研发项目（项目号：20202BBFL63050）,题目：扁刺蛾核型多角体病毒杀虫剂生产关键技术;江西省重点研发项目（项目号：20212BBF63044）,题目：豆天蛾质型多角体病毒杀虫剂关键技术研究;; 江西省科学院包干制试点示范项目（项目号：2021YSBG21013）,题目：多功能解淀粉芽孢杆菌发酵优化及其抑菌杀蚜水剂的开发应用~~

作者(Authors): 王金昌;类承凤;陈俊晖;占智高;况文东;关丽梅;杨健;李江怀;周溪;靳亮;王君;

DOI: 10.13242/j.cnki.bingduxuebao.004199

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