赵永强;吴艳虹;章秀婷;韦韬;丛丽;邵西群;

• 1:中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室

摘要(Abstract)：

貉源阿留申病毒(Raccoon dog and arctic fox amdoparvovirus,RFAV)是自然感染貉和蓝狐的新种阿留申病毒(Amdoparvovirus),为测序RFAV全基因组序列,预测分析RFAV末端发夹结构序列分子特征。本研究采用分段克隆成功获得3株长4 832nt、4 827nt、4 830nt的RFAV全基因组序列,分别命名为RFAV-Y9J、RFAV-RD15、RFAV-HS-R,利用在线软件预测RFAV末端序列二级结构,并与水貂阿留申病毒(AMDV)末端序列进行同源性比对。结果显示阿留申病毒种间、种内3’末端基因组序列保守性强,均存在116nt的Y型发夹结构;RFAV-Y9J与RFAV-RD15毒株5′末端分别存在310nt、305nt的U型发夹结构,RFAV和AMDV种内5′末端基因组序列保守性强,而种间5′末端基因组序列有较大变异。本研究首次完整测序了RFAV的3′和5′末端序列,为其他种阿留申病毒的末端序列扩增提供一种有效方法,为构建RFAV的全基因组序列感染性克隆奠定了基础。

关键词(KeyWords)： 貉源阿留申病毒;全基因组;末端发夹结构

Abstract:

Keywords:

基金项目(Foundation): 吉林省自然科学基金（20190201169JC），题目：阿留申病毒感染的宿主普调查与病毒扩张的基因变异分析;; 中国农业科学院科技创新工程专项经费（CAAS-ASTIP-2020-ISAPS）~~

作者(Author): 赵永强;吴艳虹;章秀婷;韦韬;丛丽;邵西群;

Email:

DOI: 10.13242/j.cnki.bingduxuebao.003874

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