SARS-CoV-2 Omicron变异株荧光定量PCR鉴别方法的建立及应用Establishment and Application of Fluorescence Quantitative PCR for Identification of SARS-CoV-2 Omicron Variant
牛培华;何雅青;李晓燕;马红霞;朱琳;郑宝璐;魏欣仪;李诗敏;朱灿;卢清菊;孟君;彭博;王佶;马学军;
摘要(Abstract)：
开发和评价一种用于鉴别Omicron变异株的荧光定量PCR方法。我们针对Omicron变异株S基因的两个特异性突变位点开发了一种基于荧光定量PCR的Omicron变异株分型方法（Omicron RT-qPCR）。通过测试临床样本来评价方法的特异性，通过标准品评价方法的灵敏度。Omicron RT-qPCR分型方法显示能够可靠地区分Omicron变异株和“野生型” SARS-CoV-2及Alpha、Beta、Delta变异株及甲型流感病毒。选取68例新冠疑似样本，全基因组测序（Whole genome sequencing,WGS）结果显示44例为Omicron变异株，Omicron RT-qPCR分型结果均为阳性；11例为Delta变异株，Omicron RT-qPCR分型结果均为阴性；另外，通过WGS分析未能成功分型的7例样本，通过Omicron RT-qPCR分型方法成功鉴定为Omicron BA.1和BA.2变异株。我们开发的Omicron RT-qPCR分型方法可以在普通的PCR检测实验室应用，为我国疾控系统和医疗机构及时监测SARS-CoV-2 Omicron变异株的传播提供可靠的技术支撑。
关键词(KeyWords)： SARS-CoV-2;Omicron变异株;基因分型;检测方法
基金项目(Foundation):
作者(Authors): 牛培华;何雅青;李晓燕;马红霞;朱琳;郑宝璐;魏欣仪;李诗敏;朱灿;卢清菊;孟君;彭博;王佶;马学军;
DOI: 10.13242/j.cnki.bingduxuebao.004206
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