王晓艺,李世康,寸建萍,施勇,刘莹莹,卫海燕,张勇,王雯慧,肖金波,宋洋,严冬梅,杨倩,孙强,冀天娇,许文波

• 1:安徽理工大学医学院
• 2:世界卫生组织西太平洋区脊髓灰质炎参比实验室
• 3:国家卫生和健康委员会医学病毒学和病毒病重点实验室
• 4:中国疾病预防控制中心病毒病预防控制所

摘要(Abstract)：

埃可病毒25型（Echovirus 25,E25）属于肠道病毒B组（Enterovirus B, EV-B），其在临床上可引起包括手足口病（Hand, foot, and mouth disease, HFMD），无菌性脑膜炎（Aseptic meningitis, AM），急性弛缓性麻痹（Acute flaccid paralysis, AFP）等在内的多种疾病。本文从全国HFMD监测网络选取了2009-2018年的18株E25毒株进行全基因组测序，将所得全长序列及GenBank上目前已发表的E25全长序列，以及EV-B组其它原型株全长序列分别构建全基因组和各编码区的亲缘进化树，并依据不同进化分支选取具有代表性的两株E25毒株进行重组分析。结果显示，中国大陆地区E25在P1区具有更高的亲缘关系，而P2和P3区建树中各出现了两个分支，且在每个分支上，与多个EV-B组的原型株具有较高的核苷酸同源性，提示E25可能存在多种重组模式。此外所选的两株毒株均与EV-B的其它血清型流行株在P2及P3不同区域发生重组，且重组的模式不相同，因此中国大陆地区的E25至少存在两种重组模式，并且致病力可能与基因的重组有关。通过本研究的分析，进一步的揭示中国大陆地区E25的重组特征，对其进化变异及后续的预防与治疗具有重要意义。

关键词(KeyWords)： 埃可病毒25型;全基因组;基因重组

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划“病原学与防疫技术体系研究”重点专项项目“重要急性传染病医防协同病原基因组学监测预警和溯源技术体系研究”（项目号：2022YFC2305300）,题目：急性肠道传染病病原体基因组和关键甄别位点参比数据库的建立~~

作者(Author): 王晓艺,李世康,寸建萍,施勇,刘莹莹,卫海燕,张勇,王雯慧,肖金波,宋洋,严冬梅,杨倩,孙强,冀天娇,许文波

DOI: 10.13242/j.cnki.bingduxuebao.004359

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