雷雨璇;邹瑶;孙颖;邱增钊;刘兆青;武伟华;刘慧;王昕;房师松;舒跃龙;

• 1:中山大学公共卫生学院(深圳)
• 2:暨南大学药学院
• 3:深圳市疾病预防控制中心

摘要(Abstract)：

H7N9病毒感染除引起患者呼吸道症状外,还可能导致中枢神级系统病症。血小板活化因子(Platelet activating factor,PAF)是一种生物活性磷脂,参与神经系统的部分功能。但尚未有研究讨论PAF是否参与H7N9病毒中枢神经系统疾病致病机制。本研究通过H7N9病毒体外感染小鼠小胶质细胞(BV2)和神经母瘤细胞(N2a)发现,H7N9流感病毒可以感染BV2、N2a细胞,引起细胞明显病变并使细胞活性下降;此外H7N9病毒感染BV2细胞后,PAF浓度水平明显上升,PAF乙酰水解酶(Platelet activating factor acetylhydrolase,PAF-AH)基因pafah1b1和pafah2表达水平明显下降。而N2a细胞染毒后PAF-AH基因表达水平明显上升,染毒48h后胞内PAF浓度明显下降。本研究首次将PAF与流感病毒性脑病联系在一起,提示PAF可能参与流感病毒性脑病的致病过程,可作为治疗的药物靶点进行后续研究。

关键词(KeyWords)： H7N9流感病毒;流感病毒性脑病;血小板活化因子(PAF)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（项目号：81871631），题目：PA-FAH-PAF失衡对H7N9流感病毒性脑病的作用机制研究;; 深圳市基础研究（自由探索）（项目号：JCYJ20180307102005105），题目：H7N9流感病毒性脑病的作用机制研究~~

作者(Author): 雷雨璇;邹瑶;孙颖;邱增钊;刘兆青;武伟华;刘慧;王昕;房师松;舒跃龙;

Email:

DOI: 10.13242/j.cnki.bingduxuebao.003899

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