丁凌,冷报浪,董洪英,王海燕

• 1:杭州市第三人民医院

摘要(Abstract)：

NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor pyrin domain-containing 3,NLRP3)炎症小体的激活在H1N1流感病毒感染引起肺组织损伤中起关键作用；消退素D1(Resolvin-D1,RvD1)是具有抗炎活性的脂质介质，在多种脏器损伤模型中通过抑制NLRP3炎症小体的激活减轻组织损伤及炎症反应。为了研究RvD1对H1N1感染小鼠肺组织的保护作用及机制，本研究以雄性C57BL/6小鼠为对象、通过H1N1/FM1病毒株滴鼻的方式进行H1N1感染的建模，给予RvD1腹腔注射、空白（Negative control, NC）慢病毒或NLRP3慢病毒尾静脉注射干预。检测肺指数，肺组织病理改变，肺组织中病毒载量及NLRP3、凋亡相关斑点样蛋白（Apoptosis-associated speck-like protein, ASC）、Cleaved caspase-1的表达水平，支气管肺泡灌洗液（Bronchoalveolar lavage fluid, BALF）中IL-1β、IL-18的含量。与对照组比较，模型组小鼠肺组织出现了肺泡结构损伤、炎症细胞浸润等病理改变，肺指数及肺组织中病毒载量、NLRP3、ASC、Cleaved caspase-1的表达水平、BALF中IL-1β、IL-18的含量增加（P<0.05）；与模型组比较，RvD1组小鼠肺组织的病理改变减轻，肺指数及肺组织中NLRP3、ASC、Cleaved caspase-1的表达水平、BALF中IL-1β、IL-18的含量降低（P<0.05），病毒载量无明显变化（P>0.05）；与NC慢病毒+RvD1组比较，NLRP3慢病毒+RvD1组小鼠肺组织病理改变加重，肺指数及肺组织中NLRP3、ASC、Cleaved caspase-1的表达水平、BALF中IL-1β、IL-18的含量增加（P<0.05）。以上结果表明RvD1通过抑制NLRP3炎症小体减轻H1N1/FM1感染小鼠的肺损伤。

关键词(KeyWords)： 甲型流感病毒;H1N1/FM1病毒株;消退素D1;NLRP3炎症小体;肺损伤;炎症反应

Abstract:

Keywords:

基金项目(Foundation): 杭州市科技计划引导项目（项目号：20211231Y040）,题目：Notchl介导的肺血管内皮细胞凋亡干预慢性阻塞性肺疾病的作用机制研究~~

作者(Author): 丁凌,冷报浪,董洪英,王海燕

DOI: 10.13242/j.cnki.bingduxuebao.004363

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